XB-IMG-145288
Xenbase Image ID: 145288
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Fig. 4. Immunoblot analysis of the xrds proteins. (A) The xrds
proteins are glycosylated. Preincubation of adult retinal homogenates
with N-glycosidase-F prior to SDS-PAGE results in the detection of
a lower molecular mass protein band with each antiserum. (B) The
xrds C-terminal peptide antisera are non-cross reactive. Each reacts
with a unique protein band. This reactivity can be independently
blocked by preincubating with the cognate immunizing peptide.
(C) The xrds proteins form covalent dimers. Under non-reducing
conditions, each antiserum detects a band of approximately twice the
reduced molecular mass. Size standards (in kDa) are shown at left in
A and C. Image published in: Kedzierski W et al. (1996) Copyright © 1996. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View |