XB-IMG-145289
Xenbase Image ID: 145289
Fig. 5. Immunoprecipitation analysis of xrds interactions. Triton X-
100 extracts of Xenopus retina were loaded onto columns containing
immobilized xrds36, -35, or -38 IgG fractions. After washing, bound
xrds proteins were eluted from each column with free xrds36, -35, or
-38 peptide. These eluted fractions were analyzed by western
blotting with the three xrds peptide antisera. To control for nonspecific
aggregation of outer segment membrane proteins, fractions
were also analyzed with antiserum against bovine rhodopsin (rho).
Note that in each case, the cognate peptide most efficiently eluted its
corresponding xrds protein. However, in each case the three xrds
proteins co-eluted, with no elution of rhodopsin. Image published in: Kedzierski W et al. (1996) Copyright © 1996. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View |