Xenbase Image ID: 145327

Figure 5. miR-34/449 miRNAs are required for ciliogenesis by repressing cp110a. cp110 mRNA is derepressed in TKO tracheal epithelia (n=4). Paired t-test, ns P>0.05, * P<0.05; error bars, s.e.m. b. Cp110 protein is elevated in TKO tracheal epithelia. n=3.c, d. miR-34/449 represses cp110 to regulate MCC ciliation and basal body maturation and docking. c. Co-injection of miR-34/449 and cp110 MOs rescues MCC ciliation, whereas cp110δ3′UTR overexpression phenocopies miR-34/449 morphants. cp110 MO alone also induced ciliation defects. Ac-α-tub: cilia, phalloidin-488: Actin. (Quantification: Extended Data Figure 8b.) d. Basal body maturation/docking is reestablished in miR-34/449 morphants upon cp110 knockdown. Basal bodies: Centrin4-RFP, Actin: phalloidin-488; Insets: subapical Actin meshwork. Embryos/cells analyzed: Ctrl MO (2/3), miR-34/440MOs (5/10), miR-34/449 MOs + cp110 MO (4/7). Embryos were derived from at least two females and independent fertilizations per Xenopus experiment. e. Proposed Model of regulatory role of miR34/449 during MCC ciliogenesis through direct repression of cp110. Image published in: Song R et al. (2014) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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