Xenbase Image ID: 145475

FIG. 2. Exposure to lithium during late-blastula through gastrula stages results in deletion of part or all of the forebrain. Untreated embryos or embryos treated with lithium at stages 9-12 (as indicated to the right of each row) were cultured to the tailbud stage (stage 30) and hybridized with digoxygenin-labeled X-dll3, Xwnt-8b, En-2, and XKrox-20 probes, as indicated at the top of each column. All embryos are oriented with anterior to the left of the figure and dorsal toward the top of the figure. (A) X-dll3 staining of branchial arches (arrows), otic and olfactory placodes (arrowheads), telencephalon (open black arrow), and ventral diencepha.lon (open white arrow} is indicated. (B) Arrow denotes staining of the dorsal diencephalon and arrowhead indicates staining of cells at the forebrain-midbrain boundary following hybridization with an Xwnt-8b probe. (C) Arrow indicates En-2 signal in cells at the midbrain-hindbrain boundary. (D) XKrox-20 staining of rhombomeres 3 and 5 of the hindbrain is indicated by arrowheads. Image published in: Fredieu JR et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.e Expression Phenotype decreased amount dlx5.L expression in telencephalon Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.f Expression Phenotype mislocalised wnt8b.S expression in forebrain-midbrain boundary Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.i Expression Phenotype decreased amount dlx5.L expression in diencephalon decreased amount dlx5.L expression in otic placode decreased amount dlx5.L expression in pharyngeal arch Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.j Expression Phenotype increased amount wnt8b.S expression in forebrain Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.k Expression Phenotype mislocalised en2.L expression in midbrain Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.m Expression Phenotype decreased amount dlx5.L expression in whole organism Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.n Anatomical Phenotype wholly posteriorized embryo Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.o Anatomical Phenotype wholly posteriorized embryo Expression Phenotype increased amount en2.L expression in brain Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.p Anatomical Phenotype wholly posteriorized embryo Expression Phenotype mislocalised egr2.L expression in hindbrain Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.q Anatomical Phenotype wholly posteriorized embryo Expression Phenotype decreased amount dlx5.L expression in whole organism Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.r Anatomical Phenotype wholly posteriorized embryo Expression Phenotype decreased amount wnt8b expression in whole organism Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.s Anatomical Phenotype wholly posteriorized embryo Expression Phenotype mislocalised en2.L expression in midbrain-hindbrain boundary Xla Wt + LiCl + NF29/30 (in situ hybridization) Fig.2.t Anatomical Phenotype wholly posteriorized embryo Expression Phenotype mislocalised egr2.L expression in hindbrain Larger Image Printer Friendly View

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