Xenbase Image ID: 145531

Fig. 4. Variation in enrichment within and between studies. A. Variation of fold-change with expression level at animal and vegetal poles in this study. Scatter plot of mean expression level at the enriched pole against fold change measured between the poles for the 908 enriched genes in the pooled embryo analysis. Points are color coded according to the numbers of individual embryos in which significant asymmetric expression was detected in the single embryo analysis, see Key. Genes are clearly segregated according to the consistency of detection at the single embryo level. The much greater fold-change for vegetal pole enrichment is clearly visible (note the log scale). Genes enriched in our data and previously published are highlighted (black circles), and the most enriched novel mRNAs are indicated. B. Distribution of fold-changes at either pole for individual embryos. C. Agreement of measured fold-changes between the pooled total mRNA data and the polyA+ mRNA data of Embryo 1: Spearman correlation=0.94. D. Correlation of gene enrichment between this study and earlier work. Scatter plots of enrichment fold-change on the animal–vegetal axis for genes in this study previously reported as enriched in other studies. Left panel, blastomere data (Grant et al., 2014), right panel, oocyte data (Cuykendall and Houston, 2010). Full circles: genes enriched in present study with FDR<0.05; open circles: genes not found enriched, with FDR>0.05, fold-change in our data is simple ratio of vegetal/animal pole reads. Image published in: De Domenico E et al. (2015) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page