Xenbase Image ID: 145578

Supplemental Figure 4: Variability in the MTX phenotype. i-iv) Representative frontal views of the typical facial phenotypes observed in any given concentration from 100-500uM. There are several possible reasons for this variability such as decreased folate transporter function or increased DHFR expression potentially as a result of gene amplification. We routinely saw about 25% of embryos with a very mild or normal phenotype which suggests the possibility that our frogs are carriers for a mutation in a gene that could render them less susceptible to MTX treatment. Image published in: Wahl SE et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + Methotrexate Hydrate + NF42-43 (morphology) fig.S4.ii-iv Anatomical Phenotype decreased size of the head decreased size of the intercanthal region decreased size of the mouth Larger Image Printer Friendly View

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