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Supplemental Figure 8. DHFR MO injections into one cell at the 2-cell stage show specific effects where the morpholino was present. A total of 30 embryos were injected and fixed at stage 28-30. Embryos were sectioned and labeled for H3K4me3, ph3 and cleaved caspase-3. A. Representative image of H3K4Me3 (A) and DHFR MO-FITC (A’), merge (A”). B-B”: Representative image of pH3 (B) DHFR MO FITC (B’) and merge (B”). C-C” and D-D”: Representative image of cleaved caspase-3 (C, D), DHFR MO FITC (C’), control MO FITC (D’) and merges (C”, D”). Image published in: Wahl SE et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue h3-3a.L h3.3, h3.3a, h3f3a X. laevis Sometime during NF stage 20 to NF stage 29 and 30 head casp3.2.L Apopain, CASP-3, caspase 3, caspase-3, xCPP32 X. laevis Sometime during NF stage 20 to NF stage 29 and 30 apoptotic cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dhfr MO + NF40 (immunohistochemistry) fig.S8.a^1, a^2 Anatomical Phenotype abnormal histone methylation Xla Wt + dhfr MO + NF40 (immunohistochemistry) fig.S8.b^1, b^2 Anatomical Phenotype obsolete abnormal histone phosphorylation Xla Wt + dhfr MO + NF40 (immunohistochemistry) fig.S8.c^1, c^2 Anatomical Phenotype increased apoptosis in head Larger Image Printer Friendly View

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