Xenbase Image ID: 145586

FIGURE 2. Jmjd6 interacts with Tcf7l1. A, Co-IP detection of the interaction between overexpressed Jmjd6 and Tcf7l1 in HEK293T cells. B, overexpressed Tcf7l1 precipitated endogenous JMJD6 in HEK293T cells. C, nuclear co-localization of Jmjd6 and Tcf7l1 in HEK293T cells, as detected by immunofluorescence staining. DAPI staining reveals nuclei. D and E, test of the knockdown efficiency of miJMJD6-1. Transfection of the plasmid for miJMJD6-1 did not cause a significant reduction in both the transcript (D) and protein (E) levels of JMJD6 in HEK293T cells. F and G, test of the knockdown efficiency of miJMJD6-2 and the effect of JMJD6 knockdown on the transcription of TCF7l1 and β-Catenin. Transfection of the plasmid for miJMJD6-2 resulted in a significant decrease in both the transcript (F) and protein (G) levels of JMJD6. Meanwhile, the transcription of TCF7L1 and β-Catenin was not affected in response to the efficient JMJD6 knockdown. GAPDH in (D) and (F) was used as a loading control for RT-PCR detection of the JMJD6 transcript, and β-Actin in (E) and (G) was used as a loading control for the detection of JMJD6 protein with immunoblotting. RT-: transcription without reverse transcriptase. H, detection of the effect of endogenous JMJD6 knockdown on the interaction between exogenous Jmjd6 and Tcf7l1. Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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