XB-IMG-145588
Xenbase Image ID: 145588
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FIGURE 4. Jmjd6 enhances Wnt signaling. A, domain structure and the construction of the deletion
mutants of Xenopus laevis Jmjd6a used in the study. B, subcellular localizations of the Jmjd6 mutants
revealed by immunofluorescence. Jmjd6 without the C-terminal region lost the nuclear localization and
distributed ubiquitously throughout the whole cell. C, Jmjd6 enhanced strongly β-catenin-stimulated
reporter activity, whereas the deletion mutants of Jmjd6 did not show such an enhancing effect. Error bars
represent the standard error of the mean (SEM) of four replicates. **p<0.01. NS: not significant. D,
Tcf7l1 overexpression showed strong repressive effect on the β-catenin-stimulated reporter activity,
whereas co-transfection of the plasmid for Jmjd6 led to a significant alleviation of the repressive effect.
Error bars represent the SEM of four replicates. *p<0.05, **p<0.01. E, Jmjd6 is required for the
β-catenin-stimulated transcription. In HEK293T cells, β-catenin stimulated strongly the reporter activity.
Simultaneous knockdown of endogenous JMJD6 compromised the stimulation of the reporter activity,
which was then rescued by the co-transfection of the plasmid for Xenopus laevis Jmjd6a. Error bars
represent the SEM of four replicates. *p<0.05, **p<0.01. Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |