Xenbase Image ID: 145590

FIGURE 6. Jmjd6 is required for Xenopus embryonic development. A, Co-IP detection of the interaction between Jmjd6 and Tcf7l1 in Xenopus embryos. Each 300 pg of mRNA for tagged Jmjd6 or/and Tcf7l1 was injected into 2-cell stage embryos. At gastrula stage, embryos were collected and subjected to Co-IP assays. B, design of an antisense morpholino oligonucleotide, Jmjd6MO, against both the transcripts of jmjd6a and jmjd6b. Translational start site of each transcript is underlined. C, immunoblotting detection of the knockdown efficiency of the Jmjd6MO, as compared with the standard control morpholino (ctrlMO). One nanogram of mRNA for HA-tagged Jmjd6a that contains the Jmjd6MO binding site was injected alone or co-injected together with 20 ng of either ctrlMO or Jmjd6MO into Xenopus embryos. Embryos were collected at gastrula stage and subjected to immunoblotting. D, typical Xenopus embryos after injection of ctrlMO or different doses of Jmjd6MO, showing severe developmental defect in response to Jmjd6 knockdown. The defect became stronger in response to a higher dose of injected Jmjd6MO. Embryos are shown in lateral view, with the anterior being placed to the right of each panel. E, numbers and percentages of total and defect embryos in the experiments in (D). F, different changes in the expression of genes that are involved in anterio-posterior body axis patterning in gastrula (for cer1, dkk1, gsc and chrd) and neurula (for xag2) embryos after injection of 20 ng of ctrlMO or Jmjd6MO. v: vegetal view, with the dorsal being orientated to the top of each panel; a: anterior view, with the dorsal being placed to the top. G, numbers and percentages of embryos showing different changes in gene expression after Jmjd6 knockdown. Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue cer1.L cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 X. laevis Throughout NF stage 11 dorsal marginal zone dkk1.L dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. laevis Throughout NF stage 11 dorsal marginal zone dorsal involuting marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 dorsal marginal zone chrd.L chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 11 dorsal marginal zone ag1.L ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 X. laevis Throughout NF stage 14 cement gland primordium Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + jmjd6 MO + NF26 (morphology) fig.6.d Anatomical Phenotype abnormal incomplete closing of the neural tube abnormally incomplete structure of head decreased length of anterior-posterior axis decreased size of the head Xla Wt + jmjd6 MO + NF10.5 (in situ hybridization) fig.6.f, g Expression Phenotype decreased amount cer1.S expression in upper blastopore lip decreased amount dkk1.S expression in upper blastopore lip Xla Wt + jmjd6 MO + neurula stage (in situ hybridization) fig.6.f, g Expression Phenotype decreased amount ag1.L expression in preplacodal ectoderm Larger Image Printer Friendly View

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