Xenbase Image ID: 145591

FIGURE 7. Jmjd6 mediates Tcf7l1-regulated gene transcription. A, microarray result shows that Jmjd6 knockdown in Xenopus laevis embryos enhanced the repressive effect of Tcf7l1 on the transcription of Wnt/β-catenin target genes involved in germ layer induction and patterning, but not enhance the effect on other genes. B, in Xenopus late blastula embryos, injection of the mRNA (200 pg) for dominant-negative Tcf7l1 that lacks the β-Catenin binding site led to the repression of Wnt target genes. Co-injection of Jmjd6a mRNA (1 ng) reversed the repression effect. All embryos are shown in dorsal view, with the animal pole being orientated to the top. C, numbers and percentages of embryos showing different changes in gene expression in response to injection of dnTcf7l1 and Jmjd6 mRNA, as shown in the experiments in (B). D, knockdown of endogenous Tcf7l1 in Xenopus late blastula embryos via injection of Tcf7l1MO (40 ng) upregulated the Wnt target gene, whereas simultaneous injection of Jmjd6a mRNA (1 ng) enhanced the upregulation of gene expression and even caused ectopic transcription. In the left and middle panels, embryos are shown as dorsal view, with the animal pole being placed to the top. In the right panel, animal pole is shown to view ectopic gene expression. E, numbers and percentages of embryos showing different changes in gene expression in response to injection of Tcf7l1MO and Jmjd6 mRNA, as shown in the experiments in (D). F, injection of Tcf7l1MO (40 ng) caused an increase in the expression of the Wnt target gene in Xenopus blastula embryos. However, the increase was severely weakened when Jmjd6MO (20 ng) was injected at the same time. G, numbers and percentages of embryos showing different changes in gene expression in response to injection of Tcf7l1MO and Jmjd6MO, as shown in the experiments in (F). Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sia1.L sia, Siamois, Xsia X. laevis Throughout NF stage 11 endomesoderm nodal3.1.L nr3, Xnr-3, Xnr3 X. laevis Throughout NF stage 11 upper blastopore lip Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + tcf7l1 + NF10 (microarray) fig.7.a Expression Phenotype decreased amount cer1.S expression in whole organism decreased amount chrd.1.S expression in whole organism decreased amount dkk1.L expression in whole organism decreased amount foxa4.L expression in whole organism decreased amount gsc.L expression in whole organism decreased amount meis3.L expression in whole organism decreased amount nodal1.L expression in whole organism decreased amount nodal3.1.L expression in whole organism decreased amount otx2.S expression in whole organism decreased amount sia1.L expression in whole organism decreased amount xpo1.L expression in whole organism Xla Wt + tcf7l1 + jmjd6 MO + NF10 (microarray) fig.7.a Expression Phenotype decreased amount meis3.L expression in whole organism decreased amount xpo1.L expression in whole organism Xla Wt + tcf7l1del_970-1653-myc + NF10 (in situ hybridization) fig.7.b Expression Phenotype decreased amount sia1.L expression in dorsal marginal zone Xla Wt + jmjd6 MO + tcf7l1 MO + NF12 (in situ hybridization) fig.7.d Expression Phenotype increased amount sia1.L expression in dorsal marginal zone Xla Wt + tcf7l1 MO + NF12 (in situ hybridization) fig.7.d Expression Phenotype increased amount sia1.L expression in dorsal marginal zone Xla Wt + jmjd6 MO + tcf7l1 MO + NF10 (in situ hybridization) fig.7.f Expression Phenotype mislocalised nodal3.1.L expression in blastopore increased amount nodal3.1.L expression in blastopore increased amount nodal3.1.L expression in upper blastopore lip Larger Image Printer Friendly View

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