Xenbase Image ID: 145732

Figure 9. Structural comparison of Norrin–Fz4CRD with Wnt8–Fz8CRD.(A) Superposition of Norrin (magenta)–Fz4CRD (cyan) with Wnt8 (green)–Fz8CRD (blue) (PDB ID: 4F0A). Disulphide bonds, N-linked glycans, and PAM (of Wnt8) are shown as sticks. SOS is shown as grey surface. (B) Comparison of site 1 (PAM binding) on Fz4CRD (cyan) and Fz8CRD (blue). Fz4 His69 is disease associated. (C) The Wnt8 index finger (site 2; green) structurally overlays Norrin (β1 and β2; magenta). Norrin residues associated with diseases are boxed. (D–F), Structural comparison of Fz4CRD and Fz8CRD for ligand binding. Loop I-III residues for Fz4CRD and Fz8CRD are shown as sticks. Fz8CRD residues for Wnt8 binding (site 2) are boxed in purple. Fz4 disease-associated residues are boxed. Red arrows indicate residue substitutions between Fz4CRD and Fz8CRD. Fz8CRD residues Tyr151 and Asn152 are modelled as alanines (PDB ID: 4F0A).DOI: http://dx.doi.org/10.7554/eLife.06554.026 Image published in: Chang TH et al. (2015) © 2015, Chang et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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