Xenbase Image ID: 145839

Fig. 4. In vitro binding of Celf1 to dnd1-LE involves U/G-rich elements. (A) Co-immunoprecipitation reactions were performed with Cy3-labeled full length (FL) and 5′- or 3′-deleted fragments of the dnd1-LE RNA and in vitro translated Flag-tagged proteins. The region critical for Celf1 binding is marked in light gray (nt 86-145). (B) Binding affinities of different bacterially expressed known localization factors to wild-type (wt) and a mutant (mut) version of dnd1-LE RNA that carries multiple U/C to A point mutations in the U/G-rich Celf1 binding region, were analyzed by electrophoretic mobility shift assays. The relative amount of complexed RNA is plotted against the individual protein concentrations using non-linear curve fitting; KD values for wild-type and mutant dnd1-LE RNAs are indicated. Image published in: Bauermeister D et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page