XB-IMG-145921
Xenbase Image ID: 145921
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Figure 3. Zeta-Tubulin Depletion Disrupts Basal Body Orientation and Spacing (A) Tadpole epidermal multiciliated cells in morphant and control embryos co-expressing CLAMP-GFP (green) and centrin-(RFP or BFP) (red). Clumps of basal bodies are indicated by arrowheads and insets. The scale bars represent 5 μm. (B) Quantification of mean rootlet angle from experiments as in (A). Each arrow represents one cell, where length indicates uniformity of rootlet angles in that cell. Mean cellular rootlet orientation was statistically similar between morphants and controls (mean vector angle −64.4° in controls and −74.8° in morphants; Watson-Williams). The number of cells counted is as in (C).
(C) Vector length of plots shown in (B) is significantly reduced in morphants (∗∗∗p < 0.0001; Mann-Whitney). n indicates number of cells counted, with total number of embryos in parentheses. Error bars represent the SEM. (D and E) Quantification of basal body clumping phenotype from experiments as in (A). n indicates the number of cells counted, with total number of embryos in parentheses (shown in E). (D) The distance between each basal body and its nearest neighbor is shown as average percentage of binned nearest neighbor distances for each condition. (E) Mean distance between basal bodies is shown; this is significantly reduced in morphants (∗∗∗p < 0.0001; Mann-Whitney). In (E), error bars represent the SEM.
See also Figure S4. Image published in: Turk E et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
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