Xenbase Image ID: 145975

Fig 7. Both Pkdcc1 and Pkdcc2 promote the recruitment of Dvl to the plasma membrane. (A-O) Embryos were injected with the indicated mRNAs, the ectodermal explants were extracted and Dvl.GFP localization was observed by confocal microscopy. Dvl tagged with GFP (green) is shown on the left panel, the membrane bound RFP (mbRFP, red) is shown on the middle panel and the merge pictures are shown on the right panel. (A-C) Dvl.GFP is localized in the cytoplasm of animal cap cells injected with 300 pg of dvl.GFP mRNA. (D-F) When 300 pg of dvl.GFP mRNA are co-injected with 150 pg of fz7 RNA, Dvl.GFP is recruited to the membrane. (G-I) Co-injection of 500 pg pkdcc1 RNA and 300 pg of dvl.GFP mRNA leads to membrane recruitment of Dvl.GFP as well as (J-L) the co-injection of 500 pg of pkdcc2 mRNA and 300 pg of dvl.GFP mRNA. (M-O) the same membrane localization of Dvl.GFP is observed when 300pg of dvl.GFP mRNA are co-injected with 500 pg of each pkdcc1 and pkdcc2 mRNAs. Image published in: Vitorino M et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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