Xenbase Image ID: 145976

Fig 8. Pkdcc1 and Pkdcc2 promote the recruitment of Dvl to the plasma membrane through DEP domain. (A) Schematic representation of Dvl domains involved in both Wnt canonical and non-canonical signaling pathways, and DEP.Dvl construct containing the DEP domain of Dvl fused to GFP reporter. (B-P) Embryos were injected with the indicated RNAs, the ectodermal explants were extracted and DEP.Dvl localization was observed by confocal microscopy. DEP domain tagged with GFP (green) is shown on the left panel, the membrane bound RFP (mbRFP, red) is shown on the middle panel and the merged pictures are shown in the right panel. (B-D) DEP.Dvl is localized in the cytoplasm of cells when is overexpressed alone but is recruited to the plasma membrane when co-overexpressed with fz7 (E-G). Co-injection of DEP.dvl and pkdcc1 (H-J), pkdcc2 (K-M) or both simultaneously (N-P) leads to the membrane recruitment of DEP.Dvl. Image published in: Vitorino M et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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