Xenbase Image ID: 146028

Fig. 1. Changes in intracellular calcium that occur in the dorsal ectoderm during gastrulation. Measurements start at stage 8 (5 hours postfertilisation) and end at the neurula stage (15 hours postfertilisation). A representative embryo was injected with aequorin in an apical position of the dorsal micromeres at the 8-cell stage to localise it to the dorsal ectoderm during gastrulation. (A) Profile of aequorin-generated light over time. Emitted light was recorded with a luminometer and expressed in arbitrary (arb.) units proportional to the anode current of the photomultiplier. Two components can be observed, a single slow component and multiple rapid spikes (see text). (B) Diagram to show the injection site (left) and the subsequent location of the aequorin (right; shaded zone) during gastrulation. AP, animal pole; VP, vegetal pole; V, ventral; D, dorsal. (C) Schematic illustration of the developmental time course of this aequorin-injected embryo. Image published in: Leclerc C et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

Return to previous page