Xenbase Image ID: 146028
Fig. 1. Changes in intracellular calcium that occur in the dorsal ectoderm during gastrulation.
Measurements start at stage 8 (5 hours postfertilisation) and end at the neurula stage (15 hours
postfertilisation). A representative embryo was injected with aequorin in an apical position of the
dorsal micromeres at the 8-cell stage to localise it to the dorsal ectoderm during gastrulation.
(A) Profile of aequorin-generated light over time. Emitted light was recorded with a luminometer and
expressed in arbitrary (arb.) units proportional to the anode current of the photomultiplier. Two
components can be observed, a single slow component and multiple rapid spikes (see text).
(B) Diagram to show the injection site (left) and the subsequent location of the aequorin (right; shaded
zone) during gastrulation. AP, animal pole; VP, vegetal pole; V, ventral; D, dorsal. (C) Schematic
illustration of the developmental time course of this aequorin-injected embryo.
Image published in: Leclerc C et al. (2000)
Copyright © 2000. Image reproduced with permission of the Publisher, The Company of Biologists Ltd.
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