Xenbase Image ID: 146035

Fig. 8. LTC antagonists repress the expression of the neuralising genes Zic3 and geminin. (A-C) Embryos were treated with R(+)BayK (10 mM) or nicardipine (500 mM) from stage 8 to stage 12-12.5, and then fixed and in situ hybridisation performed at stages 12 or 24. Zic3 expression is dramatically reduced (A,B) and geminin expression is lost entirely (C) in these representative embryos, when compared to the untreated controls. (D) Representative embryos demonstrating the effect of long-term and short-term nicardipine treatment on Zic3 expression. Embryos were treated for either 4 hours (from stage 8 to stage 10) or 8 hours (from stage 8 to stage 12- 12.5) with 500 mM nicardipine, and the expression pattern of Zic3 visualised at stage 24. Maximal reduction of Zic3 expression occured when the LTCs were inhibited for the entire 8 hours of gastrulation. Bars, 0.5 mm. Image published in: Leclerc C et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Gene Synonyms Species Stage(s) Tissue zic3.S xzic3, Zinc finger protein of the cerebellum 3 X. laevis Throughout NF stage 12 dorsal marginal zone zic3.S xzic3, Zinc finger protein of the cerebellum 3 X. laevis Throughout NF stage 24 central nervous system brain spinal cord gmnn.S gem, geminin X. laevis Throughout NF stage 12 ectoderm Image source: Published Larger Image Printer Friendly View

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