XB-IMG-146036
Xenbase Image ID: 146036
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Fig. 9. Intracellular
calcium increase is
required for neural
induction mediated by
attenuation of BMP
signals. Expression of the
pan-neural marker
NCAM in the animal
caps was measured by
RT-PCR. Stage 8-9
animal caps treated with
noggin (A) or dissociated
caps (B) differentiated
into neural cells
expressing NCAM,
whereas R(+)BayK
pretreated animal caps
before noggin induction
(R(+)BayK/noggin) and
BAPTA-AM loaded caps before dissociation (Dissociated+BAPTA)
show a dramatic reduction in NCAM expression. Intact cap, animal
caps not treated with noggin or nor dissociated; sibling control
embryos served as positive control (Embryo) and PCR on the same
RNA without reverse transcription was done to check the absence of
genomic DNA (-RT). ODC is a control with ornithine
decarboxylase. Image published in: Leclerc C et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View |
