Xenbase Image ID: 146071

Fig. 1. Removal of the vegetal pole cytoplasm caused an enhanced expression of a-globin in the embryo. (A) Schematic image of the depletion experiment. The vegetal pole cytoplasm was removed by pricking the embryo at about 0.3 NT. Such embryos showed a radial symmetrical shape (DAI=0), (Kao and Elinson, 1988) and expressed a large amount of a-globin. (B) Northern blot analysis showing that expression of a-globin in the cytoplasm-removed embryos (lane 4) was drastically increased in comparison with that in untreated normal embryos (lane 1). The same effect was also observed in BMP- 4 (lane 2)- or msx-1 (lane 3) -injected embryos (5 ng RNA/embryos). The expression of EF-1a served as a loading control. Image published in: Kikkawa M et al. (2001) Copyright © 2001. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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