Xenbase Image ID: 146075

Fig. 5. Effect of animal pole tissue on a-globin expression in control and exogastrula embryos. Treated embryos (B) and untreated embryos (A) were allowed to develop until stage 7 and then cultured in 1.4å~MMR as described in Materials and Methods. Such embryos were further cultured for 2 days (stage 35/36) and were used for whole-mount in situ hybridization analysis (A,B) and Northern blotting analysis (C) to detect the expression of a- globin. In exogastrula embryos (B), no expression of a-globin was observed. Scale bar in B indicates 2 mm. In addition, the expression of a-actin was not affected in the exogastrula embryos. The expression of EF-1a served as a loading control. Image published in: Kikkawa M et al. (2001) Copyright © 2001. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue hba3.L alpha globin, alpha-globin, globin alpha T3, globin T3, Ta-globin X. laevis Throughout NF stage 28 ventral blood island Image source: Published Larger Image Printer Friendly View

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