Xenbase Image ID: 146076

Fig. 6. FGF signaling is not involved in primitive blood cell differentiation in VMZ explants. (A) Experimental protocol. Two ventral blastomeres of 4-cell-stage embryos were injected with RNA, and DMZ was excised at stage 10+, and cultured for 2 days. Explants were used either for Western blot analysis (B) or for histological study (C). (B) Western blot analysis to detect a-globin production in VMZ explants. The induction of a-globin was completely suppressed in the explants injected with tBR or tALK4 RNA, but retained in the explants injected with XFD RNA. (C) Histological observation of RNA-injected explants. A tBR-injected VMZ explant contained welldifferentiated axial structures such as neural tissue (N) and muscle (M). A tALK4-injected explant contained homogenous undifferentiated cells. Blood-like cells (B) are shown in the explants injected with XFD. A thick epidermis layer with many melanin granules is also characteristic of XFD-injected explants. Image published in: Kikkawa M et al. (2001) Copyright © 2001. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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