Xenbase Image ID: 146096

Figure 1. Identification of phb1 target genes in the neural crest. (a) Neural crest explants of 300 Xenopus neurula embryos were pooled to gain samples of total RNA. The quality of total RNA was checked by calculating the RIN-value. Pools with a RIN-value > 8 were analyzed on Affimetrix GeneChip. Each experiment was performed in triplicates. To extract putative phb1 regulated genes among the roughly 40,000 spots on the GeneChip we selected those candidates which were downregulated more than two-fold in the phb1 morphants (P < 0.2) and back-regulated more than 1.5 fold (P <1.5) in the morphants co-injected with phb1 mRNA. The complete list of these 147 putative phb1 targets is found in Supporting Information Table 2. (b) The indicated morpholinos were injected together with dextran-TRITC (dex) in one animal dorsal blastomere of 8 cell stage embryos. The injected side is marked with asterisks. Embryos were fixed at stage 18 and analyzed for the expression of twist. The reduction of twist expression (white arrows) correlates with the morpholino doses. Scale bar = 400 µm. (c) and (d) Quantification of the twist expression illustrates the dose dependence and significance of reduction in the morphants. ***P < 0.005, *P < 0.05, n = number of embryos. Image published in: Deichmann C et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue twist1.L twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 18 neural crest cranial neural crest premigratory neural crest cell anterior branchial crest posterior branchial crest Image source: Published Larger Image Printer Friendly View

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