Xenbase Image ID: 146098

Figure 3. Overexpressed vangl1 upregulates twist expression, overexpressed prl3 represses twist expression. Embryos were injected in 8-cell stage in one animal dorsal blastomere with indicated amounts of mRNA. In situ hybridization was performed at stage 18 with twist probe. Asterisks mark injected side. (a) Overexpression of 500 pg vangl1 mRNA results in a clear upregulation of twist expression. (b) Statistics revealed high significance of twist upregulation. 47% of the morphants showed enhanced twist expression. (c) Overexpression of prl3 results in a downregulation of twist expression. (d) Statistics show that twist reduction is significant even at very low doses (50 pg). ***P < 0.005, **P < 0.0, n = number of embryos. Scale bar = 400 µm. Image published in: Deichmann C et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue twist1.L twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 18 neural crest cranial neural crest premigratory neural crest cell anterior branchial crest posterior branchial crest Image source: Published Larger Image Printer Friendly View

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