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Figure 5. Vangl1 and prl3 coinjection can restore twist expression in phb1 morphants. 16 ng phb1 specific antisense morpholino were co-injected with 500 pg of the indicated mRNA and dextran-TRITC (dex) in one animal dorsal blastomere of 8-cell stage embryos. (a) Twist expression was analyzed in stage 18 embryos. Asterisks mark the injected side. The reduction of twist expression was quantified in (b). ***P < 0.005, n = number of embryos. Image published in: Deichmann C et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
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