Xenbase Image ID: 146114

Figure 3. The renewal of rod photoreceptor OSs. Tadpoles expressing Opsin-Dend2 were photoconverted at 6 dpf. Retinas were dissected and imaged for green and red fluorescence 0 hour (immediately after photoconversion) (A), 4 hours (B), 8 hours (C), 1 day (D), and 2 days (E) after photoconversion. F: Tadpoles expressing P/rds-Dend2 were photoconverted at 6 dpf and the retinas were dissected and imaged for green and red fluorescence 2 days later. G,H: Tadpoles expressing Opsin-Dend2 were photoconverted at 21–22 dpf and the retinas were dissected and imaged for green and red fluorescence 2 days (G) and 6 days (H) later, respectively. The images in the mid row are for red-Dend2 fluorescence, and represented by multiple colors. The intensity-color relationship is as shown in the middle panel of (A). In the bottom row of each image panel, the intensity of green and red fluorescence was measured in ImageJ and the percentage of Dend2 fusion protein along the longitudinal axis of the OS was calculated by normalizing to the highest concentration (100%) on the axis. Scale bar = 10 μm. Image published in: Tian G et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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