Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.

Xenbase Image ID: 146124

Fig. 1. Dystroglycan depletion affects notochord morphogenesis. (A) Immunodetection of Dg on transverse cryosections from stage 13 to 37 (indicated in the top right of the images). The arrowhead in c indicates a vacuole. (B) Immunoblot of protein extracts from wild-type (WT) embryos or embryos injected with a 5-mispair control morpholino (C-MO) or Dg-MO. (C) Cryosections of embryos co-injected with ras-GFP mRNAs and C-MO (a) or with Dg-MO (b) and then immunostained with antibodies against Dg. In Dg morphants, the Dg staining is lost. (D) The top panels show the embryo phenotypes observed with C-MO or an increasing amount of Dg-MO at stage 32. (a-d) Cryosections treated with Tor70 antibodies showing a dose-dependent effect on the notochord. The arrowhead in c indicates the discontinuity in the notochord sheath. A cell containing Dg-MO (labeled with GFP, arrow and inset in c) escaped from the notochord. (d) At a high amount of Dg-MO, the sheath disappears and the characteristic structure of the notochord is lost. Dotted lines outline the neural tube (NT). (E) Notochord morphogenesis is rescued in Dg morphants through injections of mRNA encoding Dg (Dg-FL mRNA). Dotted lines outline the NT. NC, notochord; S, somite. Scale bars: 25 μm in A,C,Da-d,E; 500 μm in upper panel in D; 10 μm in Dc inset.

Image published in: Buisson N et al. (2014)

Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

dag1.Ldystroglycan, alpha-dystroglycanX.laevisSometime during NF stage 13 to NF stage 37 and 38notochord

Image source: Published

Larger Image
Printer Friendly View

Good quality Poor quality
comment [+]
Return to previous page
Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.12.0

Major funding for Xenbase is provided by grant P41 HD064556