XB-IMG-146125
Xenbase Image ID: 146125
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Fig. 2. Dystroglycan depletion affects laminin distribution and notochord morphogenesis. (A) Embryos were co-injected with ras-GFP mRNAs and C-MO or Dg-MO. Immunodetection of laminin or using the Tor70 antibody at stage 13 through to stage 25 (indicated at the top right of the images). (a-h) In Dg morphants, laminin is absent around the notochord (NC). Dotted lines outline the notochord. (g′,h′) In situ hybridization for chordin and immunodetection using Tor70 were combined at stage 25. Laminin remains absent, and the notochord shape is affected. Arrowheads in h and h′ point to two chordin-positive cells (blue in h′) that were depleted of Dg (green in h) and had not integrated in the notochord. (B,C) Embryos were co-injected with ras-GFP mRNAs with C-MO or Dg-MO. Immunodetection of fibrillin (B) and fibronectin (C) at stages 13, 15 and 25. These components of the extracellular matrix are detected in Dg morphants. (D) Immunoblot of protein extracts of wild-type (WT) or Dg-MO embryos (stage 25). In Dg-MO protein extract, Dg is undetected and the amount of laminin is comparable to that of WT. NC, notochord; NP, neural plate; S, somite; R, ratios of laminin and β-Dg to α-tubulin normalized against that of WT are shown under each lane. Scale bars: 25 μm. Image published in: Buisson N et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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