XB-IMG-146134
Xenbase Image ID: 146134
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Supplementary Figure S6. Activin induced animal cap to obtain notochord
differentiated explants in vitro.
(a-c) Two-cell stage embryos were injected with C-Mo, Dg-Mo or Dg-Mo + Dg-FL mRNAs
and animal caps were explanted. In toto analyses showed that the elongation observed in CMo
activin-treated explants is strongly inhibited by Dg depletion (Dg-Mo, 33 ng) and
importantly, is rescued to a large extent by the co-injection of 100 pg of Dg-FL mRNAs.
Scale bars: 50 µm. (d-f) Animal caps stage 28 equivalent were sectioned and Tor-70 protein
was immunodetected. Well-differentiated notochords are clearly observable in control (d) and
rescued (f) animal caps. In e, notochord structure is indistinguishable. Scale bars: 500 µm. (g,
h) The expression of the chordin gene was detected by whole-mount in situ hybridization on
control (g) and Dg-Mo explants (h). Detection of the ras-GFP, which was used as tracer of CMo
and Dg-Mo is shown in panel h. The expression of chordin is detected in control (g) and
Dg-depleted explants stage 18 equivalent (h). Scale bars: 50 µm. Image published in: Buisson N et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |
