XB-IMG-146134
Xenbase Image ID: 146134
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Supplementary Figure S6. Activin induced animal cap to obtain notochord
differentiated explants in vitro.
(a-c) Two-cell stage embryos were injected with C-Mo, Dg-Mo or Dg-Mo + Dg-FL mRNAs
and animal caps were explanted. In toto analyses showed that the elongation observed in CMo
activin-treated explants is strongly inhibited by Dg depletion (Dg-Mo, 33 ng) and
importantly, is rescued to a large extent by the co-injection of 100 pg of Dg-FL mRNAs.
Scale bars: 50 µm. (d-f) Animal caps stage 28 equivalent were sectioned and Tor-70 protein
was immunodetected. Well-differentiated notochords are clearly observable in control (d) and
rescued (f) animal caps. In e, notochord structure is indistinguishable. Scale bars: 500 µm. (g,
h) The expression of the chordin gene was detected by whole-mount in situ hybridization on
control (g) and Dg-Mo explants (h). Detection of the ras-GFP, which was used as tracer of CMo
and Dg-Mo is shown in panel h. The expression of chordin is detected in control (g) and
Dg-depleted explants stage 18 equivalent (h). Scale bars: 50 µm. Image published in: Buisson N et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher. Larger Image Printer Friendly View |