Xenbase Image ID: 146144

Fig. 2. Vangl2 and Daam1 are required for blastopore formation. (A-D) Blastopore defects in embryos depleted of Vangl2 and Daam1. Embryos were injected with control (CO) MO, Vangl2 MO or Daam1 MO, as indicated, and lacZ RNA (150 pg) as a lineage tracer. The vegetal view is shown. Dorsal is to the top. A schematic of a gastrula embryo is shown in the inset in A. (A-C) Blastopore formation (arrowhead) is inhibited in stage (st) 10+ embryos by Vangl2 MO or Daam1 MO. (D) Quantification of the effect. (E,E′) Myosin II light chain (MLC) phosphorylation at the blastopore lip (arrow) in control embryos. (F,F′) Vangl2 MO inhibits Myosin II activation (asterisk) in apically constricting cells. Scale bar: 20 µm. Image published in: Ossipova O et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue myl2.S cmh10, mlc2, XMLC2 X. laevis Throughout NF stage 10.25 blastopore lip bottle cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + vangl2 MO + NF10.25 (morphology) fig.2.b Anatomical Phenotype absent blastopore Xla Wt + daam1 MO + NF10.25 (morphology) fig.2.c Anatomical Phenotype absent blastopore Xla Wt + vangl2 MO + NF10.25 (immunohistochemistry) fig.2.f, f^1 Expression Phenotype decreased amount myl2.L expression in blastopore lip Larger Image Printer Friendly View

Return to previous page