Xenbase Image ID: 146145

Fig. 3. Roles of downstream PCP components in bottle cell formation. (A-D) Early blastopore defects in embryos expressing dominant interfering constructs for PCP and Rock pathway components. Embryos were injected with GFP, N-Daam1 or RhoA-N19 RNAs (2 ng each) or DN-Rock RNA (0.1 ng), together with lacZ RNA (0.15 ng) as lineage tracer. Vegetal view of stage (st) 10+ embryos is shown. Dorsal is to the top. (E-G) Embryos expressing N-Daam1 and RhoA-N19 display delayed blastopore closure at stage 11. Arrows in A and E indicate blastopore lip in control GFP-expressing embryos. (H) Quantification of the effect. N-Dm1, N-Daam1; N19, RhoA-N19. Image published in: Ossipova O et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + dnHsa.daam1 + NF10.25 (morphology) fig.3.b Anatomical Phenotype absent upper blastopore lip Xla Wt + dnHsa.rhoa + NF10.25 (morphology) fig.3.c Anatomical Phenotype absent upper blastopore lip Xla Wt + dnrock2 + NF10.25 (morphology) fig.3.d Anatomical Phenotype abnormal gastrulation Xla Wt + dnHsa.daam1 + NF11 (morphology) fig.3.f Anatomical Phenotype abnormally delayed closure of blastopore Xla Wt + dnHsa.rhoa + NF11 (morphology) fig.3.g Anatomical Phenotype abnormally delayed closure of blastopore Larger Image Printer Friendly View

Return to previous page