Xenbase Image ID: 146149

Fig. 7. Myosin V regulates endogenous and Shroom-mediated apical constriction. (A-C) Early embryos were injected with 2 ng of RNAs encoding GFP (A) or the carboxy-terminal tail of Myosin V fused to GFP (GFP-MyoVT, B). Blastopore defects of stage (st) 11 MyoVT embryos (B), as compared with the control (A) are shown. (C) Quantification of the results at the early and late gastrula stages. Co, control. (D,E) GFP-MyoVT alters the normal pattern of Rab11 distribution. BL, blastopore lip. (D) Transverse section of a stage 11 blastopore, stained for endogenous Rab11. The animal-vegetal (An-Vg) axis is indicated. Arrow points to Rab11 staining in bottle cells. Scale bar: 20 µm. (E,E′) Transverse section of a stage 11 defective blastopore from a MyoVT-expressing embryo. MyoVT forms cytoplasmic aggregates that colocalize with endogenous Rab11 protein. (F,G) GFP-MyoVT interferes with Shroom-mediated constriction. Scoring was carried out for embryos with strong (++) and mild (+) ectopic constriction. Image published in: Ossipova O et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rab11a.S AB11A, rab11 X. laevis Throughout NF stage 11 blastopore lip bottle cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dnmyo5b-GFP + NF10.5 (morphology) fig.7.b Anatomical Phenotype abnormally delayed closure of blastopore Xla Wt + dnmyo5b-GFP + NF10.5 (immunohistochemistry) fig.7.e, e^1 Expression Phenotype mislocalised rab11a.L expression in blastopore lip Xla Wt + dnmyo5b-GFP + NF10.5 (immunohistochemistry) fig.7.e. e^1 Expression Phenotype decreased amount rab11a.L expression in bottle cell Xla Wt + shroom1 + NF10.5 (morphology) fig.7.f Anatomical Phenotype abnormally localised ectoderm Larger Image Printer Friendly View

Return to previous page