Xenbase Image ID: 146152

Fig. 3. EZH2 interacts directly with Snail2. RNAs (1-2 ng) encoding tagged and untagged PRC2 proteins and Flag-Snail2 were injected alone or together, and proteins were extracted at gastrula stages 10-11 for IP and western blot analyses. (A) Reciprocal co-IP assay demonstrated that HA-EZH2 interacts with Flag-Snail2 in Xenopus embryos. (B) HA-EED and Flag-Snail2 do not co-precipitate. However, in the presence of EZH2 and SUZ12, HA-EED was pulled down by anti-Flag antibody, indicating that Snail2 recruited the entire PRC2 complex. (C) Purified GST-Snail2, but not GST, pulled down commercially available purified EZH2 directly. Image published in: Tien CL et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page