Xenbase Image ID: 146157

Fig. 8. Snail2 regulates EZH2 occupancy and H3K27me3 levels at the promoter of the Snail2 direct target E-cadherin during neural crest EMT. (A) Knockdown of Snail2 or EZH2 did not appreciably affect E-cad expression at early neurula stages 13-14, but resulted in increased E-cad expression at late neurula stages 19-20, when the neural crest underwent EMT to emigrate from the neural tube. Student's t-test revealed statistically significant changes in E-cad expression in morphant versus control samples at stages 19-20 (P<0.05). (B) Genomic structure around the E-cad promoter and the regions assayed in the ChIP experiments are shown at the top. Snail2-MO (20 ng) was injected into each blastomere of two-cell-stage embryos. Control and injected embryos were harvested at late neurula stages 19-20 for ChIP assay of EZH2 binding and H3K27me3 association with the E-cad promoter. Anti-GFP antibody was used as negative control. Knockdown of Snail2 reduced EZH2 occupancy and decreased H3K27me3 levels at the E-cad promoter, implying that Snail2 recruited EZH2/PRC2 to the E-cad promoter to deposit the repressive chromatin marks during EMT. Image published in: Tien CL et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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