XB-IMG-146170
Xenbase Image ID: 146170
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Fig. 5. Ezrin depletion leads to impaired actin remodelling and intracellular ciliary axoneme formation in Xenopus MCCs. (A-A‴) Increased actin accumulation (arrows) directly above the cluster of Centrin-RFP-labelled basal bodies (arrowheads) in Xenopus embryos (stage 32) injected with TB-MO ezrin (8 ng) and stained with phalloidin 488. (B-C′) TEM analysis revealed basal body migration and docking defects (arrows) and intracellular axoneme formation (arrowheads) in Xenopus embryos (stage 32) injected with TB-MO ezrin (8 ng) (C-C′) as compared with proper apically docked basal bodies (arrows) of Co-MO (8 ng) morphants (B,B′). (D-G) TEM analysis revealed that Xenopus embryos (stage 32) injected with TB-MO ezrin (8 ng) exhibit a normal ultrastructure of intracellular axonemes with nine outer microtubule doublets and two central microtubule doublets (arrowheads in E,G) and basal bodies with nine peripheral microtubule triplets (arrows in F). Scale bars: 5 μm in A‴; 1 μm in B,C,C′; 0.5 μm in B′,C′,D,G. Image published in: Epting D et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |