Xenbase Image ID: 146503

Fig. 4. NF2 depletion increases the Wnt/β-catenin signaling activity. Dual luciferase assay comparing the activities of TOP-flash reporter in cMO- and NF2 MO-injected embryos. cMO or NF2 MOs together dual luciferase reporter DNA were injected into two dorsal animal cells at the 8-cell stage, embryos were lysed at stage 15 for the detection of luciferase activities. (B) Western blotting analysis for NF2, β-catenin in the neural plate explants from cMO- or NF2 MOs-injected embryos. cMO or NF2 MOs were injected into two dorsal animal cells at the 8-cell stage and neural plates were dissected at stage 15 and subjected to western blotting analysis. GAPDH was used a loading control. (C, D) Comparison of the levels of nuclear β-catenin in control and NF2 MO-injected neural plate of Xenopus embryo at stage 15 side by side through indirect immunofluorescence analysis, indicating that the levels of nuclear β-catenin in NF2 depleted cells were higher than those in control cells. Photographs shown in panel C were acquired using a 20 × lens. The boxed area in panel C was digitally enlarged 4 times and displayed in panel (D). RLDx was used as a lineage tracer. The nuclei were counter stained with DAPI. (E) Quantitative comparison of the signal intensity of nuclear β-catenin in the neural epithelial cells in the uninjected and NF2 MOs-injected side of neural plate. p-Value was obtained through the student t-test. Image published in: Zhu X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + Nf2 MO + NF15 (immunohistochemistry) fig.4.c, d Anatomical Phenotype abnormal Wnt signaling pathway Expression Phenotype mislocalised ctnnb1.L expression in neural plate Larger Image Printer Friendly View

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