Xenbase Image ID: 146510

Figure 2. SOX18 induction of Nodal-related genes in animal caps: To compare the activity of SOX18 to SOX7 and SOX17 in the animal cap system, fertilized eggs were injected with RNAs encoding SOX7-GFP, mtSOX17β-GFP, or SOX18β-GFP (0.5 ng/embryo). At stage 8, animal caps were prepared, cultured for approximately 4 hr (when control embryos had reached stage 10/11), and then analyzed by RT-PCR (unless otherwise noted, this is the “standard” animal cap assay used throughout this work). As reported previously, SOX7-GFP induced the expression of the five mesoderm-inducing nodal-related genes Xnr1, Xnr2, Xnr4, Xnr5, and Xnr6, as well as Mixer and Endodermin (Edd), while SOX17β-GFP induced the expression of Xnr4 and Edd, but not Mixer. SOX18β-GFP induced weak expression of Xnr1, and stronger expression of Xnr2, Xnr4, Mixer, and Edd. Image published in: Zhang C et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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