Xenbase Image ID: 146511

Figure 3. SOX7 and SOX18 morpholinos: A: The sequence of the morpholinos against SOX7 and SOX18, their alignment with their target sequences, and the analogous regions of the other F-type SOX mRNAs are shown. The ability of the SOX7 morpholino to inhibit SOX7 RNA translation has been established previously (Zhang et al., 2005). B: To test the specificity of the SOX18 morpholino, fertilized eggs where injected with 0.5 ng/embryo utr-SOX18β-V5 RNA either alone or in the presence of control (CMO) or SOX18 (18MO) morpholinos (15 ng/embryo). At stage 8/9, embryonic lysates were generated and analyzed by SDS-PAGE/immunoblot using a monoclonal antiV5 antibody. The accumulation of SOX18-V5 polypeptides (marked with an asterisk) was blocked by the SOX18 morpholino, but unaffected by the control morpholino. C: Rabbit reticulocyte extracts (25 μL final volume) were programmed with 2 μg each of utr-SOX18β-V5 andSOX3-V5 RNAs either alone or together with 80 ng SOX18 morpholino. The reactions were then analyzed by SDS-PAGE and immunoblot using the monoclonal antiV5 antibody. The SOX18 morpholino completely inhibited the accumulation of SOX18-V5 polypeptide, but had no effect on SOX3V5 polypeptide accumulation. Image published in: Zhang C et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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