Xenbase Image ID: 146512

Figure 6. Direct versus indirect targets of SOX7 regulation: Fertilized eggs were injected with RNA encoding GR-SOX7-GFP (0.5 ng/embryo). Animal caps were treated in four different ways: either left in standard media (AC) with 1% ethanol (the carrier for the dexamethasone), incubated with 20 μM dexamethasone (+DEX), pretreated with 100 μg/ml emetine for 30 min and then incubated with 20 μM dexamethasone and 100 μg/ml emetine (+DEX +Eme), or incubated with 100 μg/ml emetine alone (+Eme). The presence of emetine had no apparent effect on the expression of the genes examined. In the presence of dexamethasone, the Xnrs were induced, along with Eomes, Hex, Wnt11, and Cerberus; of these genes, Xnr4, Xnr5, and Xnr6 were expressed in the presence of both dexamethasone and emetine, indicating that they are direct targets of SOX7 regulation. Image published in: Zhang C et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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