Xenbase Image ID: 146520

Fig. 5. EtOH and RA have opposed effects on gene expression. (A) Quantitative real-time PCR was employed to study the changes in gsc, Otx2 and chordin expression at st. 10.5 as a result of EtOH (2.5%), RA (1 μM) and Citral (60 μM) treatment. P < 0.05. (B) Quantitative real-time PCR analysis of Cyp26 expression as a result of EtOH and RA treatment. Whole embryos (grey) or dorsal marginal zone explants (black) were processed for RNA extraction at st. 10.5. P < 0.005. (C) Embryos were injected with the RAREZ, RA reporter plasmid, and subsequently treated with EtOH and RA. Quantitation of the reporter plasmid activity was performed (st. 10.5–11) using a chemiluminiscent substrate for ß-galactosidase. P < 0.0005. Image published in: Yelin R et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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