Xenbase Image ID: 146523

Fig. 8. Retinoic acid signaling in Spemann's organizer. The detection of RA signaling was performed using the RAREZ or RAREGFP reporter plasmids. (A–C) Embryos injected with the RAREZ plasmid and stained for ß-galactosidase activity at stages (A) 13, (B) 30 and (C) 10.5. (D) RAREZ transgenic embryo at st. 11.5 stained for ß-galactosidase activity. (E) Transgenic embryo with the RAREGFP plasmid during early/mid gastrula stages. (F) Same embryo as in (E) illuminated by epifluorescence and normal illumination to demonstrate the location of the GFP signal. The dashed line marks the position of the dorsal lip of the blastopore. (G) Quantitation of the RA signaling using the RAREZ reporter plasmid. At the onset of gastrulation, the DMZ and VMZ regions were explanted from embryos injected with the RAREZ plasmid and were processed for quantification of the ß-galactosidase activity at st. 10.5–11. The control samples were whole embryos extracts. P < 0.015. Image published in: Yelin R et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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