XB-IMG-146524
Xenbase Image ID: 146524
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Fig. 9. EtOH can rescue the teratogenic effects of ROL and RAL. The rescue assays included phenotypic, gene expression and reporter plasmid activity assays. (A–D) A combined phenotypic and gene expression assay was performed at st. 33. Embryos were treated with: (A) control, (B) 70 μM ROL, (C) 1.5% EtOH and (D) 70 μM ROL together with 1.5% EtOH. The embryos were processed for in situ hybridization with probes for en2, Pax6 and XAG1. The insets are enlargements of the head region. (E–H) Rescue analysis during gastrulation based on the gsc expression pattern. The embryos were studied at st. 10.5 after treatment with (E) control, (F) 70 μM ROL (G) 1.5% EtOH and (H) 70 μM ROL and 1.5% EtOH. (I–L) EtOH can rescue the teratogenic effects of RAL. Embryos were studied at st. 33 for head phenotype and the expression of the en2, Pax6 and XAG1 markers. (I) control, (J) 5 μM RAL, (K) 1.5% EtOH and (L) 5 μM RAL and 1.5% EtOH. (M) Embryos were injected with the RAREZ plasmid, treated with ROL (150 μM), EtOH (1.5% or 2.5%), or a combination of both. The determination of the ß-galactosidase activity was performed at st. 10.5–11. P < 0.0001. Image published in: Yelin R et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |