Xenbase Image ID: 146603

FIG. 3. Ectopically expressed Xgsk-3 disrupts expression of anterior and lateral genes. Embryos were injected in one cell at the two-cell stage with Xgsk-3FS (E) or Xgsk-3 (A–D, F–H) and b-galactosidase RNA, cultured until stage 13.5 (A) or stage 14 (B– H), and stained for b-galactosidase activity and the indicated gene expression. (A and B) XAG-1 expression in embryos viewed from the dorsal side. Anterior is facing to the left in (A) and to the right in (B), with the injected sides facing down. Note the presence of b-galactosidase activity and the absence of XAG-1 expression within the neural plate region (arrowhead) in (B). Although not apparent in the photograph, the neural folds, which mark the borders of the neural plate, are readily seen with a microscope. (C and D) Xotx2 expression on the uninjected (C) and injected (D) sides of one embryo. Note anterior (arrow) and posterior (arrowhead) regions of expression in (C). In the embryo in (D), only the anterior region of expression has expanded. (E and F) Anterior-lateral view of XANF-2 expression in embryos injected with Xgsk- 3FS (E) and Xgsk-3 (F) RNA. Note the appearance of anterior (arrow) and posterior (arrowhead) regions of expression in (E). In the embryo in (F), both the anterior and posterior regions of expression have expanded on the injected side (facing down). (G and H) Xgbx-2 expression on the uninjected (G) and injected (H) sides of one embryo. The epidermal expression is visible in (G). The neural domain of expression is mostly hidden in this view by the neural folds. The pairs of embryos in (C and D) and (G and H) are oriented with dorsal at the top and anterior facing the center. Staining for b-galactosidase activity is visible in the microscope although it is not visible in all photographs. Image published in: Pierce SB and Kimelman D (1996) Copyright © 1996. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ag1.L ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 X. laevis Throughout NF stage 13 anterior placodal area otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 13 pre-chordal neural plate hesx1.S anf2, hesx1-a, hesx1-b, rpx, Xanf, Xanf-1, XANF-2, Xanf1, Xanf2 X. laevis Throughout NF stage 13 anterior neural ridge pre-chordal neural plate border gbx2.1.S XGBX-2 X. laevis Throughout NF stage 13 neural plate Image source: Published Larger Image Printer Friendly View

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