Xenbase Image ID: 146767

Fig.1. Fluorescence intensity and volumes of CHO cells expressing EGFP. (A) Transmission image of pDP3 cells. (B) Fluorescence intensity of the cells in A, measured with the CLSM; this image is the average of two successive x-y scans taken at the z-level where the apparent cell diameters were maximal. (C) Three-dimensional surface renderings of the cells of panels A and B, from the viewpoint of the objective in the inverted microscope, and presenting for each cell an iso-intensity surface at a level ∼3% of the maximum fluorescence. (D) Mean fluorescence intensity of 485 pDP3 cells (•) and 193 non-expressing control CHO cells (○), plotted as a function of the cell volume. The green filled symbols plot the data of the cells illustrated in panels A-C. The symbols to the right give the mean±s.d. of each population, scaled relative to the mean of the pDP3 cell fluorescence. The dashed lines show the boundaries of 15 bins into which the data were pooled to examine dependencies of the estimated aqueous volume fraction on cell volume and EGFP levels. Image published in: Peet JA et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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