Xenbase Image ID: 146825

FIGURE 2. Lamin B1, B2, and A concentrations affect nuclear size in X. laevis egg extract. Similar experiments were performed as in Figure 1, using recombinant GFP-LB1, GFP-LB2, and GFP-LA rather than GFP-LB3 (see the Figure Legend for Figure 1). A) Images of representative nuclei. B) Cumulative data from 22 different X. laevis egg extracts are shown. For each data point, the cross-sectional areas of 65-890 nuclei were measured from NPC-stained nuclei, averaged, and normalized to the buffer addition control (bold horizontal line set at 1.0). The open symbols represent data for lamin proteins without a GFP tag. The error bars are SEM. All normalized nuclear area data points greater than 1.06 and less than 0.95 are statistically different from the buffer control (1.0) by at least p<0.05. Image published in: Jevtić P et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page