Xenbase Image ID: 146973

FIG. 4. Identification of proteins binding to xARH-α C terminus. A, diagram showing the arrangement of known functional regions within xARH. The clathrin box is striped, and the DφF/W motifs are shown as three solid bars in the C-terminal region. Proteins from embryo extracts that bound to the C-terminal region of xARH were affinity-purified with GST-C and resolved by SDS-PAGE and silver staining. Five proteins with molecular masses of 119, 108, 53, 48, and 35 kDa were pulled down by GST-C but not by GST alone. B, the 108-kDa band was excised from the gel and identified by quadrupole time-of-flight mass spectrometry. One spectrum matched a peptide sequence in α-adaptin. The only nonmatching residue of 13 is underlined. Image published in: Zhou Y et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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