Xenbase Image ID: 146975

FIG. 6. Dφ(F/W) motifs are required for the interaction between xARH and the AP-2 complex. A, diagram of xARH mutants fused to GST used in the analysis of the Dφ(F/W) motifs. The Dφ(F/W) motifs at positions 235, 258, and 305, respectively, were individually mutated to AAA. B, fusion proteins were used in pull-down assays to test for the binding of β2-adaptin to ARH and were analyzed subsequently by Western blotting. The nitrocellulose membrane was stained with Ponceau S to visualize the GST fusion proteins and to confirm equal loading. GST and GST-C served as the negative and positive controls, respectively. Image published in: Zhou Y et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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