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Figure 1. Expression pattern of Hspa9 in Xenopus laevis embryo. A: RT-PCR hspa9 expression in Xenopus laevis embryos from stages 3 to 40 of development with odc1 as house-keeping gene. B–H: Whole-mount ISH of hspa9. B: Early gastrula, arrow indicates the position of the blastoporal lip. C: Early neurula stage (anterior view, dorsal side up) shows hspa9 expression in neural and mesodermal territories. D,E: hspa9 is expressed in somite (s), mesoderm (m) and intermediate mesoderm (im). F: hspa9 labels the pronephros anlage (pa). G: At early tail bud stage, strong expression of hspa9 in eye (e), otic vesicle (ov), branchial arches (ba), somites and pronephros, from proximal (red arrow) to distal part (black arrow). G′: Section through the plan of dotted line in G. H,H′: Tadpole stage shows persistent hspa9 expression in the pronephros tubule. I–L: hspa9 pronephros expression was compared with pax8 and pax2 renal markers. Arrows show similar hspa9 and pax expression domains, up to the tip of the nephric duct. A strong hspa9 expression is present in the proctodeum (asterisk in I, K). Abbreviations: dt, distal tubule; g, glomus; it, intermediate tubule; n, notochord; pt, proximal tubule. Scale bars = 0.5 mm in B–H; 1 mm in I–L.Download figure to PowerPoint
