Xenbase Image ID: 147088

Figure 2. Decreased expression of hspa9 following in vivo reduction of RA signaling. A: Analysis of the GFP tracer distribution on one side of cyp26-injected embryos at neurula stage. B–I: pax8 ISH of cyp26-injected embryos at 0.15 ng (B,D,F,H) or 0.50 ng (C,E,G,I) dose was performed at st14. The otic territory (ot) and kidney field (kf) are specifically labeled by pax8 (D–G) and show a strongly reduced (F) or absent (G) pax8 positive renal field as already noticed on dorsal views (red arrows in B and C). Similar observation is made at st25 on the side of cyp26-injected embryos (H, I). J–Q: Expression of hspa9, pax8 and pax2 upon reduced RA signaling. At st24, hspa9 and pax8 are significantly reduced on the cyp26-injected side in the pronephros area (arrows, K,M). Hspa9 expression is reduced in the most anterior somites areas (white bracket) and branchial arch (*) as well (K). Similar results are obtained when compared with pax2 at late tadpole stage (O,Q). The nephric duct elongation was unaffected at the 0.15 ng dose of cyp26 injection (arrows in O,Q).Download figure to PowerPoint Image published in: Gassié L et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 16 lateral plate mesoderm neural fold anterior neural fold pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 25 lateral plate mesoderm pronephric mesenchyme otic vesicle otic placode pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 24 lateral plate mesoderm pronephric mesenchyme otic placode hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 24 somite head somite trunk somite otic placode pronephric mesenchyme lateral plate mesoderm hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 37 and 38 somite head somite trunk somite pronephric kidney pronephric nephrostome pronephric duct pronephric nephron glomus pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 37 and 38 hindbrain midbrain-hindbrain boundary otic vesicle pronephric kidney pronephric nephrostome pronephric duct early distal tubule early proximal tubule Image source: Published Larger Image Printer Friendly View

Return to previous page