Xenbase Image ID: 147091

Figure 5. Impaired hspa9 expression in Xenopus laevis disturbs tubular pronephric development. A–F: Whole-mount smp30 ISH in MoC (A–C) and Mo-injected (D–F) tadpole embryos at the dose of 17 ng. G,H: At tail bud stage, smp30 expression is decreased upon Mo34 injection (34 ng). I–L: pax8 (I,J) and pax2 (K,L) ISH confirm the impaired development of the proximal pronephric tubule after reduced hspa9 expression. M–P: Double-immunofluorescence with 3G8 (red) and 4A6 (blue) antibodies (M,O) and corresponding drawings (N,P) illustrate a simplified pronephric tubule on the Mo17-injected side of the embryo.Download figure to PowerPoint Image published in: Gassié L et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue rgn.L regucalcin, smp30, xsmp-30, xsmp30 X. laevis Throughout NF stage 35 and 36 pronephric kidney glomus proximal tubule late proximal tubule early proximal tubule proximal tubule branch rgn.L regucalcin, smp30, xsmp-30, xsmp30 X. laevis Throughout NF stage 32 pronephric kidney glomus proximal tubule late proximal tubule early proximal tubule proximal tubule branch pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 32 pronephric kidney pronephric nephrostome glomus proximal tubule early proximal tubule late proximal tubule proximal tubule branch pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 32 pronephric kidney pronephric nephrostome pronephric duct pronephric nephron glomus proximal tubule late proximal tubule early proximal tubule proximal tubule branch distal tubule early distal tubule late distal tubule hindbrain midbrain-hindbrain boundary head mesenchyme optic stalk post-anal gut Image source: Published Larger Image Printer Friendly View

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